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human epidermal growth factor receptor 2 her2 au565 cells  (ATCC)
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
Human Her2 Erbb2 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
Recombinant Human Her, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of <t>AU565</t> tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth
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Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of AU565 tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth

Journal: Photonix

Article Title: Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications

doi: 10.1186/s43074-025-00216-0

Figure Lengend Snippet: Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of AU565 tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth

Article Snippet: Human epidermal growth factor receptor 2 (HER2+) AU565 cells (ATCC CRL-2351; breast cancer) were cultured in complete RPMI 1640 cell culturing medium containing HEPES supplemented with fetal bovine serum and penicillin/streptomycin.

Techniques: Fluorescence, Imaging, Derivative Assay